Three baculovirus genes involved in late and very late gene expression: ie-1, ie-n, and lef-2
نویسندگان
چکیده
منابع مشابه
Identification and characterization of vlf-1, a baculovirus gene involved in very late gene expression.
We have identified a gene required for strong expression of the polyhedrin gene by characterizing a mutant, tsB837, of the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) which is temperature sensitive (ts) for occluded virus production at the nonpermissive temperature. Marker rescue experiments utilizing an overlapping set of AcMNPV genomic clones revealed that the gene ...
متن کاملEighteen baculovirus genes, including lef-11, p35, 39K, and p47, support late gene expression.
We report the identification of four additional genes of the Autographa californica nuclear polyhedrosis virus involved in expression from a late baculovirus promoter in transient expression assays. Three of these genes, p35, 39K, and p47, have been previously described. The role of the p35 gene product in late gene expression may be related to its ability to block apoptosis, since two other ba...
متن کاملNineteen baculovirus open reading frames, including LEF-12, support late gene expression.
A set of 18 plasmid subclones of the Autographa californica nuclear polyhedrosis virus genome, each containing an identified late expression factor gene (lef), supports expression from a late viral promoter in transient expression assays in the SF-21 cell line derived from Spodoptera frugiperda. We have constructed a further set of plasmids in which each lef open reading frame (ORF) is controll...
متن کاملTranscriptional activity of baculovirus very late factor 1.
The product of the vlf-1 (very late factor 1) gene is required for expression of very late genes during the final phase of infection. To determine whether VLF-1 functions as a transcriptional activator, VLF-1 was overexpressed and purified by affinity and cation exchange chromatography. The addition of purified protein to transcription assays containing baculovirus RNA polymerase stimulated tra...
متن کاملCharacterization of late gene expression factors lef-9 and lef-8 from Bombyx mori nucleopolyhedrovirus.
Late gene expression factors, LEF-4, LEF-8, LEF-9 and P47 constitute the primary components of the Autographa californica multinucleocapsid polyhedrovirus (AcMNPV)-encoded RNA polymerase, which initiates transcription from late and very late promoters. Here, characterization of lef-9 and lef-8, which encode their corresponding counterparts, from Bombyx mori NPV is reported. Transcription of lef...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1993
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.67.4.2149-2158.1993